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Using In Vitro Live-cell Imaging to Explore Chemotherapeutics Delivered by Lipid-based Nanoparticles
Journal JoVE
Médecine
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Journal JoVE Médecine
Using In Vitro Live-cell Imaging to Explore Chemotherapeutics Delivered by Lipid-based Nanoparticles

Using In Vitro Live-cell Imaging to Explore Chemotherapeutics Delivered by Lipid-based Nanoparticles

DOI:
10.3791/55405-v

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10:26 min

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November 01, 2017

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1Laboratory Experimental Surgical Oncology, Section Surgical Oncology, Department of Surgery,Erasmus MC

Chapitres

  • 00:05Titre
  • 00:55Plate Cells
  • 02:34Time-lapse Microscopy
  • 08:19Results: Cellular Uptake of Chemotherapeutics in Living Cells
  • 10:00Conclusion

Summary

Traduction automatique

Traduction automatique

Live-cell imaging is a powerful tool to visualize dynamic processes. The examination of fixed cells provides only static pictures, which can lead to misinterpretation and confusion about the process. This work presents a method to study uptake, drug release, and intracellular localization of liposomal nanoparticles in living cells.

Tags

In Vitro Live-cell ImagingChemotherapeuticsLipid-based NanoparticlesRecherche en cancérologieDrug DeliveryCellular UptakeReal-time Dynamic EvaluationNanoparticle ImagingImaging Chamber AssemblyCell PlatingConfocal MicroscopyIncubation UnitCell Culture Conditions

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