Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator

Adam J. Rossano; Michael F. Romero

doi:10.3791/55698

Journal

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Biologie

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用荧光基因编码的 ph 指示剂对果蝇氏小管上皮细胞内 ph 值的光学定量

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Biologie

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Journal JoVE Biologie

Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator

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用荧光基因编码的 ph 指示剂对果蝇氏小管上皮细胞内 ph 值的光学定量

DOI:

10.3791/55698-v

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11:54 min

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August 11, 2017

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Adam J. Rossano, Michael F. Romero²

¹Department of Physiology and Biomedical Engineering,Mayo Clinic College of Medicine, ²Department of Nephrology and Hypertension,Mayo Clinic College of Medicine

Chapitres

00:05Titre
00:54Dissection of Adult Drosophila Anterior Malpighian Tubules
04:32Validation of Imaging Protocol and Tubule Health
09:47Results: Quantification of Acid Extrusion in Malpighian Tubule Epithelia
10:56Conclusion

Summary

Traduction automatique

细胞离子传输通常可以通过监测细胞内 ph 值(ph 值_i)来评估。基因编码的 ph 值指标(GEpHIs)提供了细胞内 ph 值的光学定量。本议定书详细介绍了细胞内 ph 值的量化, 通过蜂窝状的前体活成像的氏管的果蝇与 pHerry, pseudo-ratiometric 基因编码的 pH 指标。