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Immunologie et infection

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用于炎症反应的新内皮调节因子的筛选测定

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Immunologie et infection

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Journal JoVE Immunologie et infection

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

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用于炎症反应的新内皮调节因子的筛选测定

DOI:

10.3791/55824-v

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12:50 min

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September 15, 2017

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María Ángeles Higueras, Lidia Jiménez-García, Sandra Herranz, Sonsoles Hortelano*¹, Alfonso Luque*¹

¹Pharmacological Therapies Unit, Research Institute for Rare Diseases,Institute of Health Carlos III

Chapitres

00:05Titre
00:58Endothelial Cell Culture and Tissue Culture Conditions
02:42Evaluate Endothelial Factor Released on Leukocyte Activation by Adhesion Assays
04:12Leukocyte Adhesion Assay
07:42Test Endothelial Activation by Leukocyte Endothelium Co-adhesion Assay
09:59Results: Activation Parameters of LPS-stimulated Endothelial Cells and Influence on Leukocyte Behavior
11:52Conclusion

Summary

Traduction automatique

血管内皮严密控制白细胞的招募。不充分的白细胞外渗导致人类炎症性疾病。因此, 寻找新的调节元素内皮活化是必要的设计改进治疗炎症性疾病。在这里, 我们描述了一个全面的方法来表征新的内皮调节剂, 可以改变白细胞贩运在炎症。

Tags

Endothelial Regulators Inflammatory Response Screening Assay Inflammatory Disorders Leukocyte Recruitment Cytokines Chemokines Adhesion Molecules RT-qPCR Wes MLEC-04 Cells Lipopolysaccharide (LPS)Ccl5 Cxcl1 Cxcl10 E-selectin VCAM-1 ICAM-1

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