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JoVE Journal
Immunology and Infection

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Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
 

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Article DOI: 10.3791/55824-v 12:50 min September 15th, 2017
September 15th, 2017

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Summary

Vascular endothelium tightly controls leukocyte recruitment. Inadequate leukocyte extravasation contributes to human inflammatory diseases. Therefore, searching for novel regulatory elements of endothelial activation is necessary to design improved therapies for inflammatory disorders. Here, we describe a comprehensive methodology to characterize novel endothelial regulators that can modify leukocyte trafficking during inflammation.

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Keywords: Endothelial Regulators Inflammatory Response Screening Assay Inflammatory Disorders Leukocyte Recruitment Cytokines Chemokines Adhesion Molecules RT-qPCR Wes MLEC-04 Cells Lipopolysaccharide (LPS) Ccl5 Cxcl1 Cxcl10 E-selectin VCAM-1 ICAM-1
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