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An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers
 

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers

Article DOI: 10.3791/55833-v • 06:34 min • December 1st, 2017
December 1st, 2017 •
*1, *1, 1, 1, 1, 1,2,3, 1,4
1Applied Microbiology Research, Department of Biomedicine, University of Basel, 2Department of Biosystems Science and Engineering, ETH Zurich, 3Swiss Institute of Bioinformatics, 4Clinical Microbiology, University Hospital Basel
* These authors contributed equally

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résumé
Automatic Translation

The presented protocols describe how to perform a hemagglutination inhibition assay to quantify influenza-specific antibody titers from serum samples of influenza vaccine recipients. The first assay determines optimal viral antigen concentrations by hemagglutination. The second assay quantifies influenza-specific antibody titers by hemagglutination inhibition.

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Keywords: Hemagglutination Inhibition Assay Influenza-specific Antibody Titers Vaccine-mediated Immunity Neutralizing Antibodies Mouse Serum Culture Supernatants Antigen Solution RDE Treated Serum Samples Antiserum Serial Dilutions Red Blood Cell Solution Incubation Time Hemagglutination
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