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Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
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Biologie
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Journal JoVE Biologie
Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

DOI:
10.3791/55911-v

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09:32 min

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September 10, 2017

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, ,
1National Health and Environmental Effects Research Laboratory,U.S. Environmental Protection Agency, 2Oak Ridge Institute for Science and Education at U.S. Environmental Protection Agency

Summary

Traduction automatique

Traduction automatique

A capillary-based immunoassay using a commercial platform to measure target proteins from total protein preparations is demonstrated. In addition, assay parameters of exposure time, protein concentration, and antibody dilution are optimized for a cell culture model system.

Tags

Capillary ElectrophoresisImmunoassayAutomated ProcedureProtein QuantitationCell CultureBiofluidsWestern BlotSample PreparationMaster MixDenaturing ConditionsAntibody Dilutions

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