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Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
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Journal JoVE Neurosciences
Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

DOI:
10.3791/55936-v

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09:51 min

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January 01, 2018

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, , ,
1The Institutes of Brain Science, the State Key Laboratory of Medical Neurobiology, the Collaborative Innovation Center for Brain Science,Fudan University

Chapitres

  • 00:05Titre
  • 01:05Preparation of Acute Hippocampal Slices
  • 02:21Modifications of the Carbogenation for the aCSF Recycling of Small Reservoirs
  • 03:08Recording Synaptic Responses in a Submersion Slice Chamber
  • 07:28Results: Representative Recordings of Activity-dependent Potentiaation of Synaptic Transmission Using Outflow-carbogenation and Reservoir-carbogenation
  • 08:08Conclusion

Summary

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Traduction automatique

This protocol describes the stabilization of the oxygen level in a small volume of recycled buffer and methodological aspects of recording activity-dependent synaptic plasticity in submerged acute hippocampal slices.

Tags

Synaptic PlasticityAcute Hippocampal SlicesRecycling-perfusion-submersion ChamberCarbogenationField Potential RecordingsNeuroplasticitySynaptic TransmissionMemory FormationOxygen StabilizationAcute Slice PreparationVibratomeHippocampal FormationRecycling SystemCarbogen Supply

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