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Pulling Membrane Nanotubes from Giant Unilamellar Vesicles
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Journal JoVE Biologie
Pulling Membrane Nanotubes from Giant Unilamellar Vesicles

Pulling Membrane Nanotubes from Giant Unilamellar Vesicles

DOI:
10.3791/56086-v

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06:26 min

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December 07, 2017

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, , ,
1Laboratoire Physico Chimie Curie, Institut Curie,PSL Research University, CNRS UMR168, 2Department of Genetics and Complex Diseases, T. H. Chan School of Public Health,Harvard Medical School, 3Department of Cell Biology,Harvard Medical School, 4Sorbonne Universités,UPMC University Paris 06, 5Center for Studies in Physics and Biology,The Rockefeller University

Chapitres

  • 00:05Titre
  • 00:50Preparation of the Tube-pulling Experimental Chamber
  • 02:32Membrane Nanotube Pulling
  • 05:12Results: Representative Nanotube Formation Analyses
  • 05:58Conclusion

Summary

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Traduction automatique

Many proteins in the cell sense and induce membrane curvature. We describe a method to pull membrane nanotubes from lipid vesicles to study the interaction of proteins or any curvature-active molecule with curved membranes in vitro.

Tags

Membrane NanotubesGiant Unilamellar VesiclesProtein-membrane InteractionsEndocytosisIntracellular TraffickingLipid MembranesProtein-shaping MembranesElectroformationBeta-caseinAspiration MicropipetteInjection MicropipetteStreptavidin-coated Beads

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