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Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo
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Journal JoVE Médecine
Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo

Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo

DOI:
10.3791/56199-v

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07:35 min

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May 08, 2018

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, , ,
1Department of Ophthalmology,University Medical Center of the Johannes Gutenberg University Mainz

Chapitres

  • 00:05Titre
  • 01:05Mouse Retina Isolation
  • 03:00Perfusion Chamber Retina Mounting
  • 04:31Retinal Arteriole Preparation and Experimentation
  • 05:58Results: Representative Functional Analyses
  • 06:49Conclusion

Summary

Traduction automatique

Traduction automatique

Many vision-threatening ocular diseases are associated with dysfunctional retinal microvessels. Therefore, the measurement of retinal arteriole responses is important to investigate the underlying pathophysiological mechanisms. This article describes a detailed protocol for mouse retinal arteriole isolation and preparation to assess the effects of vasoactive substances on vascular diameter.

Tags

Retinal ArteriolesRetina PreparationMouse RetinaRetinal Blood FlowVideo MicroscopyRetinal Blood Vessel ReactivityOphthalmic ArteryKrebs-Henseleit BufferVannas ScissorsPrecision TweezersCorneaChoroidSclera

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