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Developmental Biology

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JoVE Journal Developmental Biology
Multi-Photon Time Lapse Imaging to Visualize Development in Real-time
 

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

Article DOI: 10.3791/56214-v • 10:13 min • August 9th, 2017
August 9th, 2017 •
1, 1
1Department of Ophthalmology and Visual Sciences, Kellogg Eye Center, University of Michigan

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A combination of the advanced optical techniques of laser scanning microscopy with long wavelength multi-photon fluorescence excitation was implemented to capture high-resolution, three-dimensional, real-time imaging of neural crest migration in Tg(sox10:EGFP) and Tg(foxd3:GFP) zebrafish embryos.

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Multi-photon Time Lapse Imaging Visualization Development Real-time Migrating Neural Crest Cells Zebrafish Embryos Confocal Microscopy Multi-photon Lasers Tissue Penetration Phototoxicity Embryo Collection Breeding Tanks Reverse Osmosis Water Mating Embryos Egg Collecting Screen Petri Dishes Embryo Medium
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