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Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
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Journal JoVE Recherche en cancérologie
Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

DOI:
10.3791/56418-v

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09:09 min

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November 10, 2017

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, , ,
1Department of Biochemistry and Molecular Biology,University of Arkansas for Medical Sciences, 2Department of Experimental Therapeutics,University of Texas MD Anderson Cancer Center

Chapitres

  • 00:05Titre
  • 00:41Assembling the Elutriation System
  • 02:55Priming the Elutriation System
  • 04:56Introduction of Cell Sample into the Elutriation System and Collection of Fractions
  • 06:56Results: Evaluation of Centrifugal Elutriation-based Sorting of Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases
  • 08:21Conclusion

Summary

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This protocol describes the use of centrifugal elutriation to separate primary acute lymphoblastic leukemia cells into different cell cycle phases.

Tags

Primary Acute Lymphoblastic LeukemiaCell Cycle PhasesCentrifugal ElutriationCell SeparationCancer TherapeuticsAnti-cancer CompoundsCell IsolationPhysical PropertiesCell CycleStrobe AssemblyRotorElutriation ChamberRotating Seal AssemblyFlow SystemBufferVariable Speed PumpSterilization5% Bleach

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