Journal
/
Biologie
/
Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth

DOI:
10.3791/56497-v

•

07:28 min

•

November 24, 2017

•

, ,
1Division of Biological Sciences,University of Missouri

Chapitres

  • 00:05Titre
  • 00:45Culturing, Growth, and Target-specific Staining of A. tumefaciens Strains
  • 02:21Imaging of A. tumefaciens Cells
  • 04:54Epifluorescence and Time-lapse Microscopy
  • 05:41Results: Cells Growth of Live Labeled Microbial Cells Imaged with Fluorescence Microscopy
  • 06:47Conclusion

Summary

Traduction automatique

Traduction automatique

Understanding the function of essential processes in bacteria is challenging. Fluorescence microscopy with target-specific dyes can provide key insights into microbial cell growth and cell cycle progression. Here, Agrobacterium tumefaciens is used as a model bacterium to highlight methods for live cell imaging for characterization of essential processes.

Tags

Live Cell Fluorescence MicroscopyBacterial Cell GrowthEssential ProcessesAgrobacterium TumefaciensFluorescent ReagentsDMSO OrangeDAPIAgarose PadsTime-lapse Imaging

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/56497?article_type=v

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
check_url/fr/56497?article_type=v

Time-lapse Imaging of Mitosis After siRNA Transfection
check_url/fr/56497?article_type=v

Cell Electrofusion Visualized with Fluorescence Microscopy
check_url/fr/56497?article_type=v

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
check_url/fr/56497?article_type=v

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast
check_url/fr/56497?article_type=v

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis
check_url/fr/56497?article_type=v

Visualizing Stromule Frequency with Fluorescence Microscopy
check_url/fr/56497?article_type=v

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
check_url/fr/56497?article_type=v

Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy
check_url/fr/56497?article_type=v

Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software

Read Article