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An In Vivo Duo-color Method for Imaging Vascular Dynamics Following Contusive Spinal Cord Injury
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
An In Vivo Duo-color Method for Imaging Vascular Dynamics Following Contusive Spinal Cord Injury

An In Vivo Duo-color Method for Imaging Vascular Dynamics Following Contusive Spinal Cord Injury

DOI:
10.3791/56565-v

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09:25 min

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December 31, 2017

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, , , , , , ,
1Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, and Department of Neurological Surgery,Indiana University School of Medicine, 2Program in Medical Neuroscience, Stark Neurosciences Research Institute,Indiana University School of Medicine, 3Norton Neuroscience Institute,Norton Healthcare, 4Department of Human Anatomy & Histoembryology, School of Basic Medical Sciences,Fudan University, 5Department of Neurological Surgery,University of Louisville School of Medicine

Chapitres

  • 00:05Titre
  • 01:22External Jugular Vein Catheterization
  • 03:09Spine Stabilization, C5 – C7 Laminectomy, and Two-photon (2P) Imaging Window Installation
  • 05:02First Fluorescent Dye Injection and Baseline Imaging
  • 06:25Louisville System Apparatus (LISA) Device-induced C7 Contusive Injury, Second Fluorescent Dye Injection, and Post-injury Imaging
  • 07:26Results: Representative In Vivo Vascular Dynamic Imaging Following Contusive Spinal Cord Injury
  • 08:27Conclusion

Summary

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We introduce an in vivo imaging method using two different fluorescent dyes to track dynamic spinal vascular changes following a contusive spinal cord injury in adult Sprague-Dawley rats.

Tags

In VivoDuo-colorTwo-photon ImagingVascular DynamicsContusive Spinal Cord InjuryNeuroprotective StrategiesVascular RepairSprague Dawley RatExternal Jugular VeinCatheterSpinal Cord Injury

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