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The Ingestion of Fluorescent, Magnetic Nanoparticles for Determining Fluid-uptake Abilities in Insects
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Journal JoVE Biologie
The Ingestion of Fluorescent, Magnetic Nanoparticles for Determining Fluid-uptake Abilities in Insects

The Ingestion of Fluorescent, Magnetic Nanoparticles for Determining Fluid-uptake Abilities in Insects

DOI:
10.3791/56619-v

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07:03 min

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December 20, 2017

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, , , , , , ,
1Department of Biological Sciences,Kent State University at Stark, 2Department of Mathematical Sciences,Clemson University, 3Liquid Crystal Institute,Kent State University, 4Brookhaven National Laboratory

Chapitres

  • 00:05Titre
  • 00:38Insect Species, Preparation of Solutions, and Feeding Station Setup
  • 01:43Feeding Protocol
  • 03:05Dissections
  • 04:31Testing for Ingested Nanoparticles
  • 05:24Results: A Relationship Between Feeding Pore Size and Feeding Conduit Size
  • 06:28Conclusion

Summary

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Traduction automatique

Fluid-feeding insects have the ability to acquire minute quantities of liquids from porous surfaces. This protocol describes a method to directly determine the ability for insects to ingest liquids from porous surfaces using feeding solutions with fluorescent, magnetic nanoparticles.

Tags

Fluorescent Magnetic NanoparticlesFluid-feeding InsectsInsect Mouth PartsInsect Feeding MechanismsBlue Bottle FliesHouse FliesCabbage ButterfliesSucrose SolutionFeeding StationNylon Net FiltersMembrane FiltersMicroscope SlidesManipulatorFeeding StageFilter PaperInsect Pin

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