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Recherche en cancérologie
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Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
Journal JoVE
Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts

DOI:
10.3791/57011-v

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07:18 min

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June 13, 2019

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, , , ,
1Department of Pathology,Icahn School of Medicine at Mount Sinai

Chapitres

  • 00:04Titre
  • 00:44Sarcoma Tissue Sample Processing and Patient-derived Xenograft (PDX) Formation
  • 02:32Tumor-initiating Cell (TIC) Sorting and Sarcosphere Formation
  • 05:11Results: Representative Human Leukocyte Antigen (HLA)-1-Negative TIC Characterization
  • 06:42Conclusion

Summary

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We describe a detailed protocol for the isolation of tumor-initiating cells from human sarcoma patient-derived xenografts by fluorescence-activated cell sorting, using human leukocyte antigen-1 (HLA-1) as a negative marker, and for the further validation and characterization of these HLA-1-negative tumor-initiating cells.

Tags

Tumor-initiating CellsSarcomaPatient-derived XenograftsHLA-IEpigenetic DownregulationCancer Stem CellsImmune EscapeSingle Cell SuspensionNOD Scid Gamma MouseBasement Membrane MatrixHistological Analysis

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