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Cancer Research

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JoVE Journal Cancer Research
Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma
 

Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma

Article DOI: 10.3791/57012-v • 06:11 min • April 20th, 2018
April 20th, 2018 •
*1, *2, 3, 4, 3, 1, 1, 1, 3, 1,3
1Department of Otorhinolaryngology, Johannes-Gutenberg University Medical Center, 2Department of Otorhinolaryngology, Technical University of Munich Medical Center, 3Department of Otorhinolaryngology, Ludwig-Maximilian-University Medical Center, 4Department of Otorhinolaryngology, University of Goettingen Medical Center
* These authors contributed equally

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résumé
Automatic Translation

We describe the evolution of a spheroid-based, three-dimensional in vitro model that enables us to test the current standard of experimental therapy regimens for head and neck squamous cell carcinoma on cell lines, aiming at evaluating therapy susceptibility and resistance on primary cells from human specimens in the future.

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Therapy Testing Spheroid-based 3D Cell Culture Head And Neck Squamous Cell Carcinoma In Vitro Method Experimental Therapy Regimens Tumor Growth Radiation Treatment Chemo Radiation Treatment Primary Tumor Cells Three-dimensional Spheroid Formation Sabina Schwenk-Zieger Technician Tumor Specimen Sterile Surface Collagenase 1 And 2 Incubate Falcon Cell Strainer HBSS T75 Cell Culture Flask Subconfluency Microscope Cell Count Ultra-low Adhesion 96-well Plate
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