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Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
This content is Free Access.
1Cryo Electron Microscopy, Centre for Advanced Materials,Universität Heidelberg, 2Heidelberg Karlsruhe Research Partnership (HEiKA), 3Cryo Electron Microscopy, BioQuant,Universitätsklinikum Heidelberg, 4Institute for Automation and Applied Computer Science,Karlsruhe Institute of Technology (KIT), 5Carl Zeiss Microscopy GmbH, 6Electron Microscopy Core Facility,Universität Heidelberg
Chapitres
- 00:04Titre
- 01:16Ultramicrotome and Sample Setup
- 03:04Ultramicrotome Sectioning
- 04:21Hierarchical Imaging in the SEM
- 07:33Registration of the SEM Image Stack
- 08:39Results: Multimodal Hierarchical Imaging of Serial Sections of Plant Roots
- 10:06Conclusion
This protocol targets specific cells in tissue for imaging at nanoscale resolution using a scanning electron microscope (SEM). Large numbers of serial sections from resin-embedded biological material are first imaged in a light microscope to identify the target and then in a hierarchical manner in the SEM.
