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Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model: The Thymus and Parathyroid Glands Organogenesis Paradigm
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Biologie du développement
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Journal JoVE Biologie du développement
Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model: The Thymus and Parathyroid Glands Organogenesis Paradigm

Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model: The Thymus and Parathyroid Glands Organogenesis Paradigm

DOI:
10.3791/57114-v

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13:43 min

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June 17, 2018

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,
1Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina,Universidade de Lisboa

Chapitres

  • 00:00Titre
  • 03:05Isolation of Quail Embryonic Region Containing the Presumptive Territory of Thymic and Parathyroids Rudiments
  • 05:49Organotypic Assay: In Vitro Culture of the Embryonic Region Containing the Presumptive Territory of Thymic and Parathyroids Rudiments
  • 08:17Chorioallantoic Membrane (CAM) Assay: Grafting of Cultured Explants onto CAM, Ectopic Organ Formation after 10 Days in CAM
  • 10:59Representative Results of the Two-Step Approach
  • 13:08Conclusion

Summary

Traduction automatique

Traduction automatique

This article provides an updated approach to the classical quail-chicken chimera system to study organ formation, by combining novel in vitro and in ovo experimental procedures.

Tags

Two-step ApproachOrgan FormationAvian ModelThymusParathyroid GlandsOrganogenesisIn Vitro ManipulationIn Ovo GraftingOrganotypic SystemChorioallantoic MembranePharmacological AgentsSignaling PathwaysGene ExpressionMorphological FeaturesBiologie du développement

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