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Chemistry

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Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
 

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Article DOI: 10.3791/57133-v • 11:48 min • April 24th, 2018
April 24th, 2018 •
*1,2, *3,4,5, 3, 6, 6, 3,4, 3,4,5, 1,2,4, 1,7
1Center for Free Electron Laser Science, DESY, 2Department of Physics, University of Hamburg, 3Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, University of Hamburg, 4The Hamburg Center for Ultrafast Imaging, University of Hamburg, 5Integrated Biology Infrastructure Life-Science Facility at the European XFEL (XBI), 6European Molecular Biology Laboratory, EMBL c/o DESY, 7Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry
* These authors contributed equally

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This protocol describes in detail how to fabricate and operate microfluidic devices for X-ray diffraction data collection at room temperature. Additionally, it describes how to monitor protein crystallization by dynamic light scattering and how to process and analyze obtained diffraction data.

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Microfluidic Chips In Situ Crystal X-ray Diffraction In Situ Dynamic Light Scattering Serial Crystallography Protein Crystallization X-ray Transparent Chips Synchrotron Beamlines X-ray Diffraction Data Collection PDMS Base Curing Agent Master Mold Aluminum Foil Vacuum Desiccator PDMS Mold Epoxy Mold Casting
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