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Immunologie et infection
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Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans
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Immunologie et infection
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Journal JoVE Immunologie et infection
Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans

Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans

DOI:
10.3791/57171-v

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08:24 min

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February 27, 2018

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, , , , , ,
1Department of Biology,Middle Tennessee State University, 2DRVision Technologies

Chapitres

  • 00:04Titre
  • 00:50Capsule Induction and Yeast Cell Staining
  • 03:14Image Acquisition
  • 04:27Capsule Measurement
  • 06:33Results: Representative Capsule Measurement
  • 07:58Conclusion

Summary

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Traduction automatique

The polysaccharide capsule is the primary virulence factor in Cryptococcus neoformans, and its size correlates with strain virulence. Capsule diameter measurements are used in phenotypic testing and to gauge therapeutic efficacy. Here a standard method of capsule induction is presented, and two methods of staining and measuring diameter are compared.

Tags

Cryptococcus NeoformansCapsule DiameterCapsule StainingIndia InkCalcofluor-whiteAlexa Fluor 488Capsule MeasurementVirulenceAutomated Analysis

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