Journal
/
Biochimie
/
Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
Journal JoVE
Biochimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biochimie
Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

DOI:
10.3791/57177-v

•

09:33 min

•

March 20, 2018

•

1Department of Biochemistry,Charles University

Chapitres

  • 00:04Titre
  • 02:01Incubation of Carcinogen and Drugs with DNA in the Presence of Enzymatic Systems
  • 03:04Isolation of the DNA from the Incubate
  • 04:3032P-postlabeling for Detection of DNA Adduct Formation
  • 05:04TLC Separation of Adducted Deoxyoligonucleotides
  • 05:43Results: Study of the Potency of Metabolically Active Ellipticine Drug in Generating Covalent DNA Adducts
  • 07:33Conclusion

Summary

Traduction automatique

Traduction automatique

Evaluating the potency of environmental chemicals and drugs, to be enzymatically bioactivated to intermediates generating covalent DNA adducts, is an important field in the development of cancer and its treatment. Methods are described for compound activation to form DNA adducts, as well as techniques for their detection and quantification.

Tags

Keywords: Covalent DNA AdductsEnzymatic ActivationCarcinogensDrugs32P-postlabelingCytochrome P450BiotransformationCancer DevelopmentMolecular MechanismsDNA DamageToxification

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/57177?article_type=v

Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
check_url/fr/57177?article_type=v

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
check_url/fr/57177?article_type=v

Real-time Tracking of DNA Fragment Separation by Smartphone
check_url/fr/57177?article_type=v

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
check_url/fr/57177?article_type=v

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
check_url/fr/57177?article_type=v

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
check_url/fr/57177?article_type=v

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
check_url/fr/57177?article_type=v

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils
check_url/fr/57177?article_type=v

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
check_url/fr/57177?article_type=v

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Read Article