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Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry
Journal JoVE
Médecine
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Journal JoVE Médecine
Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

DOI:
10.3791/57319-v

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07:32 min

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March 06, 2018

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, , , , ,
1Department of Internal Medicine,MUMC, 2CARIM,MUMC, 3Department of Immunology and Biochemistry, Biomedical Research Institute,Hasselt University, 4Department of Pathology,MUMC

Chapitres

  • 00:04Titre
  • 01:06Stromal Vascular Fraction (SVF) Adipose Tissue (AT) Isolation
  • 02:42SVF Staining and Flow Cytometry Analysis
  • 04:50Results: Representative Visceral AT Immune Cell Abundance
  • 06:33Conclusion

Summary

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This article describes a method to analyze immune cell content of adipose tissue by isolation of immune cells from adipose tissue and subsequent analysis using flow cytometry.

Tags

Flow CytometryStromal Vascular FractionImmune CellsPhenotypingMetabolic ResearchObesityInflammationCell IsolationCell Staining

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