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Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue
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Biochimie
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Journal JoVE Biochimie
Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue

Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue

DOI:
10.3791/57376-v

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14:55 min

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April 07, 2018

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, , , ,
1Département de chimie et biochimie,Université de Moncton, 2Département de biologie,Université de Moncton

Chapitres

  • 00:00Titre
  • 00:47Introduction
  • 01:33Calibration
  • 03:01Sample Preparation
  • 05:39Mitochondrial Oxygen Consumption Experiment
  • 09:31Representative results
  • 13:57Conclusion

Summary

Traduction automatique

Traduction automatique

In this paper, a method to measure oxygen consumption using high-resolution respirometry in permeabilized thoraxes of Drosophila is described. This technique requires a minimal amount of tissue compared to the classic mitochondrial isolation technique and the results obtained are more physiologically relevant.

Tags

DrosophilaMitochondrial RespirationHigh-resolution RespirometryPermeabilized FibersElectron Transport SystemSubstrate OxidationUncouplerInhibitors

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