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Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue
 

Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue

Article DOI: 10.3791/57376-v 14:55 min April 7th, 2018
April 7th, 2018

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résumé

In this paper, a method to measure oxygen consumption using high-resolution respirometry in permeabilized thoraxes of Drosophila is described. This technique requires a minimal amount of tissue compared to the classic mitochondrial isolation technique and the results obtained are more physiologically relevant.

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Keywords: Drosophila Mitochondrial Respiration High-resolution Respirometry Permeabilized Fibers Electron Transport System Substrate Oxidation Uncoupler Inhibitors
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