Journal
/
Biologie
/
Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation
Journal JoVE
Biologie
Author Produced
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation

Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation

DOI:
10.3791/57422-v

•

08:52 min

•

July 15, 2018

•

, , ,
1Plant Molecular and Cellular Biology Program,University of Florida, 2Department of Biology,University of Florida, 3Genetics Institute (UFGI),University of Florida, 4Alkali Soil Natural Environmental Science Center,Northeast Forestry University, 5Interdisciplinary Center for Biotechnology Research (ICBR),University of Florida

Chapitres

  • 00:02Titre
  • 01:19Preparation of Enriched Stomata
  • 03:14Protein Extraction and SDS-PAGE Separation
  • 06:14Results: Stomata Tape-Peel Allows for the Preparation of High Quality Enriched Stomata Suitable for Conducting Physiological, Biochemical and Molecular Assays
  • 07:38Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes a method of preparing enriched stomatal guard cells that is useful for physiological and other biological studies.

Tags

StomataGuard CellsEnrichmentIsolationTranscriptomicsProteomicsMetabolomicsBrassicaceaeArabidopsisScotch TapeCell Wall DigestionPhenol ExtractionProtein Precipitation

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/57422?article_type=v

MALDI Sample Preparation: the Ultra Thin Layer Method
check_url/fr/57422?article_type=v

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
check_url/fr/57422?article_type=v

Western Blotting: Sample Preparation to Detection
check_url/fr/57422?article_type=v

An Improved Method for Accurate and Rapid Measurement of Flight Performance in Drosophila
check_url/fr/57422?article_type=v

Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging
check_url/fr/57422?article_type=v

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
check_url/fr/57422?article_type=v

Sample Preparation for Mass Cytometry Analysis
check_url/fr/57422?article_type=v

An Alternative Approach for Sample Preparation with Low Cell Number for TEM Analysis
check_url/fr/57422?article_type=v

Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples
check_url/fr/57422?article_type=v

Miniaturized Sample Preparation for Transmission Electron Microscopy

Read Article