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Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy
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Chapitres
- 00:04Titre
- 01:02Imaging of the Artery Using TPEM
- 02:47Diameter and Wall Thickness Measurement
- 03:33Imaging the Collagen and Elastin Microarchitecture
- 05:10Image Analysis Internal Elastic Lamina Branching Angles
- 05:51Image Analysis – Collagen Fiber Straightness
- 07:46Results: Measurement of the Microstructural Changes in Adventitial Collagen and the Internal Elastic Lamina in Response to Vessel Pressure
- 08:48Conclusion
We describe simultaneous mechanical testing and 3D-imaging of the arterial wall of isolated, live human resistance arteries, and Fiji and Ilastik image analyses for the quantification of elastin and collagen spatial organization and volume densities. We discuss the use of these data in mathematical models of arterial wall mechanics.
