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JoVE Journal Neuroscience
Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain
 

Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain

Article DOI: 10.3791/57511-v • 11:20 min • May 7th, 2018
May 7th, 2018 •
1, 2, 1,2, 1,3
1Department of Physiology, David Geffen School of Medicine, University of California Los Angeles, 2Department of Neurology, David Geffen School of Medicine, University of California Los Angeles, 3Department of Neurobiology, David Geffen School of Medicine, University of California Los Angeles

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Potassium ions contribute to the resting membrane potential of cells and extracellular K+ concentration is a crucial regulator of cellular excitability. We describe how to make, calibrate and use monopolar K+-selective microelectrodes. Using such electrodes enables the measurement of electrically evoked K+ concentration dynamics in adult hippocampal slices.

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Keywords: Potassium Ion Selective Microelectrodes Tissue Slices Adult Brain Potassium Ion Homeostasis Borosilicate Glass Capillaries Silanization Microelectrode Fabrication DDS Silanization Solution Microelectrode Puller Potassium Ion Concentration Measurement
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