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一种快速、定量的后向基因组转化和变异的多肽映射方法
Journal JoVE
Génétique
This content is Free Access.
Journal JoVE Génétique
A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
DOI:

09:10 min

May 22, 2018

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Chapitres

  • 00:05Titre
  • 00:51Mapping of Peptides using PoGoGUI
  • 02:36Visualizing in the Integrative Genomics Viewer
  • 03:57Mapping Peptides Identified Through a Custom Variant Database to a Reference Genome
  • 04:44Mapping Peptides from Multiple Files Using PoGoGUI
  • 05:48Generating a Track Hub from Mapped Peptides and Visualizing a Track Hub in the UCSC Genome Browser
  • 06:50Results: Post-translational Modification and Varient Enabled Mapping of Peptides to Genomes
  • 08:21Conclusion

Summary

Traduction automatique

在这里, 我们提出了 proteogenomic 工具的弹跳和协议的快速, 数量, 后平移修改和变种启用映射的肽识别通过质谱的参考基因组。该工具用于集成和可视化 proteogenomic 和个人蛋白质组学的研究, 并与正交基因学数据相衔接。

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