Journal
/
Biologie
/
Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation

Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation

DOI:
10.3791/57649-v

•

06:38 min

•

May 03, 2018

•

, , ,
1Department of Molecular, Cell, and Developmental Biology,University of California, Santa Cruz

Chapitres

  • 00:05Titre
  • 01:06Urine Depletion and Bladder Rinsing
  • 02:40Plasmid Delivery
  • 05:16Results: Successful Delivery of pCAG-GFP Plasmid to the Mouse Bladder Urothelium
  • 06:13Conclusion

Summary

Traduction automatique

Traduction automatique

We describe a novel method for the delivery of DNA plasmid into the urothelial cells of mouse bladder in vivo through urethra catheterization and electroporation. It offers a fast and convenient way for generating autochthonous mouse models of bladder diseases.

Tags

Plasmid DNA DeliveryBladder UrotheliumElectroporationGene OverexpressionGenome EditingBladder CancerGene TherapyAnesthesiaCatheterPBS WashingDNA PlasmidTrypan BlueVertical Incision

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/57649?article_type=v

Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
check_url/fr/57649?article_type=v

Transformation of Plasmid DNA into E. coli Using the Heat Shock Method
check_url/fr/57649?article_type=v

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation
check_url/fr/57649?article_type=v

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
check_url/fr/57649?article_type=v

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
check_url/fr/57649?article_type=v

Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development
check_url/fr/57649?article_type=v

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation
check_url/fr/57649?article_type=v

A Novel Ex vivo Culture Method for the Embryonic Mouse Heart
check_url/fr/57649?article_type=v

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
check_url/fr/57649?article_type=v

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Read Article