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A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury
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Neurosciences
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Journal JoVE Neurosciences
A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury

A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury

DOI:
10.3791/57727-v

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09:08 min

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September 13, 2018

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, ,
1Institute of Medical Science,University of Toronto, 2Department of Surgery,University of Toronto, 3Institute of Biomaterials and Biomedical Engineering,University of Toronto

Chapitres

  • 00:04Titre
  • 00:45Minimal Spinal Cord Injury (SCI) Surgery
  • 04:01Spinal Cord Isolation and Neurosphere Culture
  • 07:18Results: Representative Neurosphere Analyses Following Minimal SCI
  • 08:24Conclusion

Summary

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Here, we demonstrate the performance of a minimal spinal cord injury model in an adult mouse that spares the central canal niche housing endogenous neural stem cells (NSCs). We show how the neurosphere assay can be used to quantify activation and migration of definitive and primitive NSCs following injury.

Tags

Neural Stem CellsSpinal Cord InjuryNeurosphere AssayEndogenous Neural Precursor CellsRegenerative MedicineMouse ModelSurgical Technique

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