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Biochemistry

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JoVE Journal Biochemistry
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
 

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

Article DOI: 10.3791/57750-v • 08:58 min • July 5th, 2018
July 5th, 2018 •
*1, *1,2, 1, 1, 1,2,3
1Program in Molecular Medicine, The Hospital for Sick Children Research Institute, 2Department of Biochemistry, University of Toronto, 3Department of Immunology, University of Toronto
* These authors contributed equally

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résumé
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We present approaches for the biophysical and structural characterization of glycoproteins with the immunoglobulin fold by biolayer interferometry, isothermal titration calorimetry, and X-ray crystallography.

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Glycoproteins Immunoglobulin Fold X-ray Crystallography Biophysical Techniques Function Of Protein N-linked Glycans Antibiotics Targeting Glycoprotein Structural Characterization Biophysical Characterization Hong Cui Research Project Coordinator Cell Transfection Centrifugation Filtration Ni-NTA Column Chromatography System Wash Buffer Elution Buffer Gradient Centrifugal Filtration Device
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