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Rapid, Scalable Assembly and Loading of Bioactive Proteins and Immunostimulants into Diverse Synthetic Nanocarriers Via Flash Nanoprecipitation
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Rapid, Scalable Assembly and Loading of Bioactive Proteins and Immunostimulants into Diverse Synthetic Nanocarriers Via Flash Nanoprecipitation

Rapid, Scalable Assembly and Loading of Bioactive Proteins and Immunostimulants into Diverse Synthetic Nanocarriers Via Flash Nanoprecipitation

DOI:
10.3791/57793-v

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06:57 min

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August 11, 2018

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, ,
1Interdisciplinary Biological Sciences,Northwestern University, 2Department of Biomedical Engineering,Northwestern University

Chapitres

  • 00:04Titre
  • 00:57Assemble PEG-bl-PPS Nanocarriers via Hand-Powered Flash Nanoprecipitation
  • 04:18Fabricate Polymersomes via Flash Nanoprecipitation (FNP) Using the Custom-Made High-Speed Syringe Pump
  • 05:27Results: Monodisperse Polymersome Formation and Loading
  • 06:12Conclusion

Summary

Traduction automatique

Traduction automatique

Nanomaterials provide versatile mechanisms of controlled therapeutic delivery for both basic science and translational applications, but their fabrication often requires expertise that is unavailable in most biomedical laboratories. Here, we present protocols for the scalable fabrication and therapeutic loading of diverse self-assembled nanocarriers using flash nanoprecipitation.

Tags

Flash NanoprecipitationNanocarriersBioactive ProteinsImmunostimulantsPolymer SynthesisImpingementCIJ MixerNanostructuresDrug DeliverySterile ConditionsHydrophobic CargoHydrophilic CargoPolymer Vesicles

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