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JoVE Journal Genetics
High-Resolution Comparison of Bacterial Conjugation Frequencies
 

High-Resolution Comparison of Bacterial Conjugation Frequencies

Article DOI: 10.3791/57812-v • 05:18 min • January 10th, 2019
January 10th, 2019 •
1,2,3, 3, 1
1Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 2Department of Bioscience, Graduated School of Science and Technology, Shizuoka University, 3Japan Collection of Microorganisms, RIKEN BioResource Research Center

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résumé
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With an aim to understand the behaviors of various bacterial conjugative DNA elements under different conditions, we describe a protocol for detecting differences in conjugation frequency, with high resolution, to estimate how efficiently the donor bacterium initiates conjugation.

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Keywords: Bacterial Conjugation Plasmid DNA Transfer Most Probable Number Conjugation Frequency High-resolution Comparison Microbiology Plasmid Transconjugants
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