Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium <em>Myxococcus xanthus</em>

Dominik Schumacher; Lotte S&#0248;gaard-Andersen

doi:10.3791/57860

/

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus

Journal JoVE
Biochimie

This content is Free Access.

Journal JoVE Biochimie

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus

DOI:

10.3791/57860-v

•

11:45 min

•

June 20, 2018

•

Dominik Schumacher, Lotte Søgaard-Andersen

¹Department of Ecophysiology,Max Planck Institute for Terrestrial Microbiology

Chapitres

00:04Titre
00:39Growth and Preparation of M. xanthus Microscopy Samples
02:41Microscope Set-up and Time-lapse Acquisition
07:37Generation of Time-lapse Movies and Image Alignment
09:16Results: Fluorescence Time-lapse Imaging of Myxococcus xanthus
11:01Conclusion

Summary

Traduction automatique

Bacterial cells are spatially highly organized. To follow this organization over time in slow growing Myxococcus xanthus cells, a set-up for fluorescence live-cell imaging with high spatiotemporal resolution over several generations was developed. Using this method, spatiotemporal dynamics of important proteins for chromosome segregation and cell division could be determined.

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus

•

•

•

Chapitres

Summary

Traduction automatique

Tags

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Imaging of Extracellular Vesicles by Atomic Force Microscopy

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Read Article