Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Long-term In Vivo Tracking of Inflammatory Cell…
JoVE Journal
Immunology and Infection

Ce contenu est en libre accès.

JoVE Journal Immunology and Infection
Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae
 

Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae

Article DOI: 10.3791/57871-v • 09:26 min • June 14th, 2018
June 14th, 2018 •
1,2, 1, 3, 1,4
1School of Biochemistry, Biomedical Sciences, University of Bristol, 2School of Cellular and Molecular Medicine, Biomedical Sciences, University of Bristol, 3MRC Centre for Inflammation Research, University of Edinburgh, Queens Medical Research Institute, 4School of Physiology, Pharmacology, and Neuroscience, Biomedical Sciences, University of Bristol

Chapitres

résumé
Automatic Translation

Here we present a protocol for live-imaging wound repair and the associated inflammatory response at high spatio-temporal resolution in vivo. This method utilizes the pupal stage of Drosophila development to enable long-term imaging and tracking of specific cell populations over time and is compatible with efficient RNAi-mediated gene inactivation.

Tags

Keywords: In Vivo Tracking Inflammatory Cell Dynamics Drosophila Pupae Live Imaging Wound Healing Innate Immunity RNAi Gene Inactivation Pupal Stage Hemocyte Pupae Collection Pupal Casing Dissection Mounting Heptane Glue
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter