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Journal / Medicine / An In Vitro Approach to Photodynamic Therapy
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An In Vitro Approach to Photodynamic Therapy
 

An In Vitro Approach to Photodynamic Therapy

Article DOI: 10.3791/58190-v • 04:53 min • August 17th, 2018
August 17th, 2018 •
1,2, 1,2,3
1Department of Dermatology, University of California, Davis, 2Dermatology Service, Sacramento VA Medical Center, 3Department of Dermatology, State University of New York, Downstate Medical Center

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résumé
Automatic Translation

Photodynamic therapy (PDT) is a medical procedure that involves incubation of an exogenously applied photosensitizer (PS) followed by visible light photoactivation to induce apoptosis. We present an in vitro PDT protocol designed to simulate PDT that may be used to study differences in PS incubation and light treatment parameters.

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Keywords: Photodynamic Therapy PDT In Vitro Photosensitizer 5-ALA Blue Light Irradiation Cell Culture Cell Plating Temperature Control Fluence Cell Detachment Flow Cytometry
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