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Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart
 

Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

Article DOI: 10.3791/58448-v • 11:45 min • January 7th, 2019
January 7th, 2019 •
1, 1,2
1Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, 2Department of Anesthesiology, University of Nebraska Medical Center

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résumé
Automatic Translation

The overall goal of this article is to standardize the protocol for the isolation, characterization, and differentiation of cardiac stem cells (CSCs) from the adult mouse heart. Here, we describe a density gradient centrifugation method to isolate murine CSCs and elaborated methods for CSC culture, proliferation, and differentiation into cardiomyocytes.

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Keywords: Cardiac Stem Cells CSCs Adult Mouse Heart Isolation Characterization Differentiation Cardiovascular Disease Heart Failure Cardiac Stem Cell Therapy Cost-effective Ham's Balanced Salt Solution Collagenase II Tissue Digestion Cell Suspension
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