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定量自动放射法测定维沃脑蛋白合成区域速率

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Neurosciences

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Journal JoVE Neurosciences

Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo

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定量自动放射法测定维沃脑蛋白合成区域速率

DOI:

10.3791/58503-v

•

11:01 min

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June 28, 2019

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R. Michelle Saré, Anita Torossian, Michael Rosenheck, Tianjian Huang, Carolyn Beebe Smith

¹Section on Neuroadaptation and Protein Metabolism, National Institute of Mental Health,National Institutes of Health

Chapitres

00:04Titre
00:43Femoral Vein and Artery Catheterization
04:46Tracer Administration and Collection of Blood Samples
06:07Analysis of Plasma Samples to Determine Concentrations of Leucine and L-[1-¹⁴C]leucine
07:07Quantitative Autoradiography
07:58Image Analysis and Computation of Regional Rates of Cerebral Protein Synthesis (rCPS)
09:02Results: Effects of Anisomycin on Rates of Cerebral Protein Synthesis
09:57Conclusion

Summary

Traduction automatique

蛋白质合成是细胞的关键生物过程。在大脑中,它是自适应变化所必需的。测量完整大脑中蛋白质合成率需要仔细的方法学考虑。这里我们介绍了L-[1-14C]-亮细胞定量自射方法,用于测定体内脑蛋白合成的区域速率。

Tags

Keywords: Quantitative Autoradiographic Method Regional Rates Cerebral Protein Synthesis In Vivo Brain Protein Synthesis Quantitative Awake Behaving Animal Autoradiographic Technique Femoral Artery Femoral Vein Surgical Procedure Suture Catheter Heparinized Saline

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