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Fluorimetric Techniques for the Assessment of Sperm Membranes
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Biologie du développement
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Journal JoVE Biologie du développement
Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

DOI:
10.3791/58622-v

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08:58 min

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November 28, 2018

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1Animal Sperm Research Center, Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem, 2Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem

Chapitres

  • 00:04Titre
  • 00:55Sperm Sample Preparation
  • 02:03Technique #1: Simultaneous Assessment of Sperm Membranes Using Multiple Flourescent Probes
  • 04:34Technique #2: Assessment of Sperm Membranes with Ready-to-use Kits and Flow Cytometry
  • 06:58Results: Evaluation of Semen Quality
  • 08:16Conclusion

Summary

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Here, we present methodologies to evaluate spermatozoan membrane integrity, a cellular feature associated with sperm fertilization competence. We describe three techniques for the fluorimetric assessment of sperm membranes: simultaneous staining with specific fluorescent probes, fluorescence microscopy, and advanced sperm-dedicated flow cytometry. Examples of combining the methodologies are also presented.

Tags

Fluorimetric TechniquesSperm MembranesFluorescent ProbesFluorescence MicroscopyFlow CytometrySperm Fertilization PotentialSperm Plasma MembraneAcrosome MembraneMitochondrial Membrane PotentialNKM BufferDAPIFITC-PSAJC-1PIEpifluorescence Microscopy

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