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JoVE Journal Developmental Biology
Fluorimetric Techniques for the Assessment of Sperm Membranes
 

Fluorimetric Techniques for the Assessment of Sperm Membranes

Article DOI: 10.3791/58622-v • 08:58 min • November 28th, 2018
November 28th, 2018 •
1,2, 1,2
1Animal Sperm Research Center, Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, 2Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem

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résumé
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Here, we present methodologies to evaluate spermatozoan membrane integrity, a cellular feature associated with sperm fertilization competence. We describe three techniques for the fluorimetric assessment of sperm membranes: simultaneous staining with specific fluorescent probes, fluorescence microscopy, and advanced sperm-dedicated flow cytometry. Examples of combining the methodologies are also presented.

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Keywords: Fluorimetric Techniques Sperm Membranes Fluorescent Probes Fluorescence Microscopy Flow Cytometry Sperm Fertilization Potential Sperm Plasma Membrane Acrosome Membrane Mitochondrial Membrane Potential NKM Buffer DAPI FITC-PSA JC-1 PI Epifluorescence Microscopy
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