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1Hotchkiss Brain Institute,University of Calgary, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine,University of Calgary, 3Alberta Children’s Hospital Research Institute,University of Calgary, 4Department of Clinical Neurosciences, Cumming School of Medicine,University of Calgary
Chapitres
- 00:04Titre
- 00:56Dissociating Tissue (Day 1)
- 03:46Isolating Viable and Healthy Cells (Day 1)
- 04:46GEM (Gel Bead in Emulsion) Generation and Barcoding (Day 1)
- 06:17NCBI’s GEO and SRA Submissions
- 07:34Results: Bioinformatics Analysis of Single Cell Transcriptomics
- 09:35Conclusion
This protocol describes the general processes and quality control checks necessary for preparing healthy adult mammalian single cells for droplet-based, high throughput single cell RNA-Seq preparations. Sequencing parameters, read alignment, and downstream single-cell bioinformatic analysis are also provided.
