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1Hotchkiss Brain Institute,University of Calgary, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine,University of Calgary, 3Alberta Children’s Hospital Research Institute,University of Calgary, 4Department of Clinical Neurosciences, Cumming School of Medicine,University of Calgary
Chapitres
- 00:04Titre
- 00:56Dissociating Tissue (Day 1)
- 03:46Isolating Viable and Healthy Cells (Day 1)
- 04:46GEM (Gel Bead in Emulsion) Generation and Barcoding (Day 1)
- 06:17NCBI’s GEO and SRA Submissions
- 07:34Results: Bioinformatics Analysis of Single Cell Transcriptomics
- 09:35Conclusion
Denne protokol beskriver de generelle processer og kvalitetskontrol kontrol nødvendig for at forberede droplet-baseret, høj overførselshastighed enkelt celle RNA-Seq præparater sunde voksne pattedyr enkelt celler. Sekventering parametre, tilbydes Læs justering og downstream encellede bioinformatic analyse også.
