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1Hotchkiss Brain Institute,University of Calgary, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine,University of Calgary, 3Alberta Children’s Hospital Research Institute,University of Calgary, 4Department of Clinical Neurosciences, Cumming School of Medicine,University of Calgary
Chapitres
- 00:04Titre
- 00:56Dissociating Tissue (Day 1)
- 03:46Isolating Viable and Healthy Cells (Day 1)
- 04:46GEM (Gel Bead in Emulsion) Generation and Barcoding (Day 1)
- 06:17NCBI’s GEO and SRA Submissions
- 07:34Results: Bioinformatics Analysis of Single Cell Transcriptomics
- 09:35Conclusion
Dit protocol worden de algemene procedures beschreven en kwaliteitscontrole controles nodig voor gezonde volwassen één zoogdiercellen voorbereiden druppel gebaseerde, hoge doorvoer eencellige RNA-Seq preparaten. Sequencing parameters, worden Lees uitlijning en downstream eencellige bioinformatic analyse ook geleverd.
