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Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
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Chapitres
- 00:04Titre
- 00:46Mitochondria and Lysosome Labeling
- 02:27Flow Cytometric Analysis
- 04:31Results: Representative Mitochondria and Lysosome Detection
- 05:51Conclusion
This article illustrates a powerful method to quantify mitochondria or lysosomes in living cells. The combination of lysosome- or mitochondria-specific dyes with fluorescently conjugated antibodies against surface markers allows the quantification of these organelles in mixed cell populations, like primary cells harvested from tissue samples, by using multicolor flow cytometry.
