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Bioengineering

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JoVE Journal Bioengineering
Lucifer Yellow - A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier
 

Lucifer Yellow - A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier

Article DOI: 10.3791/58900-v • 06:22 min • August 19th, 2019
August 19th, 2019 •
1, 1, 2, 1
1Department of Pharmaceutical, Administrative and Social Sciences, Duquesne University, 2Department of Pharmaceutical Sciences, University of Kentucky

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résumé
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We present a fluorescence assay to demonstrate that Lucifer Yellow (LY) is a robust marker to determine the apparent paracellular permeability of hCMEC/D3 cell monolayers, an in vitro model of the human blood-brain barrier. We used this assay to determine the kinetics of a confluent monolayer formation in cultured hCMEC/D3 cells.

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Keywords: Lucifer Yellow Paracellular Permeability Blood-brain Barrier Cell Model Tight Junctions Apparent Permeability Cell Plating Collagen Coating HCMEC/D3 Cells Cell Confluency Lucifer Yellow Assay Transport Buffer Fluorescence Measurement
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