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Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
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Biologie du développement
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Journal JoVE Biologie du développement
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

DOI:
10.3791/58975-v

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07:37 min

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June 11, 2019

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, ,
1Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute,Stanford University School of Medicine, 2Department of Developmental Biology,Stanford University School of Medicine

Chapitres

  • 00:04Titre
  • 00:45Preparation of Differentiation Media
  • 02:04Seed hPSCs onto Plates at Defined Densities for Differentiation
  • 04:39Differentiation of hPSCs into Endodermal Cells and Liver Progenitors
  • 05:37Results: Differentiating Human Pluripotent Stem Cells into Liver Cells
  • 06:58Conclusion

Summary

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This protocol details a monolayer, serum-free method to efficiently generate hepatocyte-like cells from human pluripotent stem cells (hPSCs) in 18 days. This entails six steps as hPSCs sequentially differentiate into intermediate cell-types such as the primitive streak, definitive endoderm, posterior foregut and liver bud progenitors before forming hepatocyte-like cells.

Tags

Human Pluripotent Stem CellsLiver CellsDifferentiationRegenerative MedicineLiver Bud ProgenitorsHepatocyte-like CellsDevelopmental Signaling PathwaysHigh PurityIMDMPVACDM2CDM3CDM4CDM5Small MoleculesGrowth FactorsMatrixHPSCDissociation Agent

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