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Genetics

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JoVE Journal Genetics
Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
 

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

Article DOI: 10.3791/58981-v • 06:08 min • January 25th, 2019
January 25th, 2019 •
1, 2, 1, 3, 3, 4,5, 1, 1
1MRC Laboratory of Molecular Biology, 2Department of Molecular and Cell Biology, University of Connecticut, 3Cancer Research UK Beatson Institute Glasgow, 4MRC Laboratory for Molecular Cell Biology, University College London, 5Department of Cell and Developmental Biology, University College London

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Dictyostelium discoideum is a popular model organism to study complex cellular processes such as cell migration, endocytosis, and development. The utility of the organism is dependent on the feasibility of genetic manipulation. Here, we present methods to transfect Dictyostelium discoideum cells that overcome existing limitations of culturing cells in liquid media.

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Keywords: Dictyostelium Discoideum Genetic Engineering Bacterial Selection Growth On Bacteria Exochromosomal Plasmid Transfection Microscopy Klebsiella Aerogenes Sorensen Buffer LB Medium Optical Density H40 Electroporation Buffer SM Medium Agar Plate Tissue Culture Dish Cell Transfer Centrifugation DNA Transformation
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