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Cancer Research

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JoVE Journal Cancer Research
A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager
 

A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager

Article DOI: 10.3791/59042-v • 09:17 min • February 2nd, 2019
February 2nd, 2019 •
1,2, 1,2, 1,2,3, 1,2
1Medical Genetics, Hunter Medical Research Institute, 2Priority Research Centre for Cancer Research, Innovation and Translation, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, 3Pathology North, John Hunter Hospital

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The current protocol describes an integrated method investigating cancer cell migration and invasion on a single platform in real-time, providing an easily reproducible and time-efficient option to study cell mobility and morphology.

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Migration Invasion Workflow Automated Live-cell Imager Cancer Cell Mobility Metastasis Cell Movement Invasive Capacity Tumor Cells Integrated Method Real Time Reproducible Time-efficient Cell Mobility Pathology Extra-cellular Matrix Scratch Time Seeding Density Incubation Period Cell Monolayer Migration Assay 96-well Plate Live Cell Imager Schedule Scans Plate Type Scan Setup Scan Type
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