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平滑肌细胞近系追踪小鼠晚期动脉粥样硬化病变细胞成分的定量分析

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Médecine

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Journal JoVE Médecine

Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

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平滑肌细胞近系追踪小鼠晚期动脉粥样硬化病变细胞成分的定量分析

DOI:

10.3791/59139-v

•

09:06 min

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February 20, 2019

•

Sidney Mahan, Mingjun Liu, Richard A. Baylis³, Delphine Gomez⁴

¹Heart, Lung, Blood and Vascular Medicine Institute,University of Pittsburgh, ²Robert M. Berne Cardiovascular Research Center,University of Virginia, ³Department of Biochemistry and Molecular Genetics,University of Virginia, ⁴Division of Cardiology,University of Pittsburgh School of Medicine

Chapitres

00:04Titre
00:36Brachiocephalic Artery (BCA) Harvest Preparation
01:56BCA Exposure and Harvest
03:05Immunofluorescent Staining
05:04Confocal Microscopy and Single Cell Counting
07:00Results: Representative Characterization of the Effects of IL-1beta Inhibition on the Cellular Composition of Artherosclerotic Lesions
08:33Conclusion

Summary

Traduction automatique

我们提出了一个标准化的方案来表征晚期小鼠动脉粥样硬化病变的细胞组成, 包括动物解剖、组织包埋、切片、染色和腕头动脉分析的系统方法从动脉粥样硬化平滑肌细胞谱系追踪小鼠。

Tags

Keywords: Quantitative Analysis Cellular Composition Advanced Atherosclerotic Lesions Smooth Muscle Cell Lineage-tracing Immunofluorescent Staining Phenotypic Characterization Qualitative And Quantitative Analyses Cell Types Brachiocephalic Artery Harvest Gravity Perfusion System Paraformaldehyde Fixation Carotid Artery Subclavian Artery Aortic Arch

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