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Xylem Water Distribution in Woody Plants Visualized with a Cryo-scanning Electron Microscope
 

Xylem Water Distribution in Woody Plants Visualized with a Cryo-scanning Electron Microscope

Article DOI: 10.3791/59154-v • 10:47 min • June 20th, 2019
June 20th, 2019 •
1, 2, 3, 4, 5, 6
1Department of Plant Ecology, Forestry and Forest Products Research Institute (FFPRI), 2Kansai Research Center, Forestry and Forest Products Research Institute (FFPRI), 3Department of Wood Properties and Processing, Forestry and Forest Products Research Institute (FFPRI), 4Faculty of Agriculture, Kyushu University, 5Department of Bacteriology, University of Wisconsin, 6Research Faculty of Agriculture, Hokkaido University

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Observing the water distribution within the xylem provides significant information regarding water flow dynamics in woody plants. In this study, we demonstrate the practical approach to observe xylem water distribution in situ by using a cryostat and cryo-SEM, which eliminates artifactual changes in the water status during sample preparation.

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Xylem Water Distribution Woody Plants Cryo-scanning Electron Microscope Water Regimes Seasonal Variation Freeze/thaw Cycles Wet Wood Cavitation Biotic Stresses Cryo-SEM Aerotome Micrographs Sampling Procedure Black Plastic Bag Water Potential
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